RPI YPD Agar 250g Laboratory Grade Natural Protein Media Y20080-250.0

Description

Product Overview

RPI YPD Agar 250 g is a laboratory‑grade agar medium formulated to support the rapid and consistent growth of a wide range of yeast and bacterial strains. The medium combines 20 g/L peptone, 20 g/L dextrose, and 10 g/L yeast extract, providing a rich source of nitrogen, carbon, and growth factors essential for robust cell proliferation. Packaged in a compact 250 g pouch, the product meets the dimensional specifications of 3.5 × 0.29 × 3.5 inches and weighs 8.82 ounces, making it easy to store and handle in busy laboratory environments.

The formulation follows the classic YPD recipe widely used in microbiology, ensuring compatibility with existing protocols and media preparation equipment. Because the ingredients are of analytical grade, the agar delivers low background fluorescence and minimal contaminants, which is critical for downstream applications such as enzyme assays, genetic transformation, and metabolite analysis. The product is not discontinued by the manufacturer, and its best‑seller rank of #9 in the Lab Natural Proteins category reflects strong demand among research institutions and biotech companies.

Each batch of RPI YPD Agar undergoes rigorous quality control testing, including sterility verification, pH consistency, and nutrient concentration analysis, to guarantee reproducible performance across multiple experiments. The agar solidifies at standard laboratory temperatures, forming a clear, uniform surface that facilitates colony counting and morphological observation. The product’s shelf life extends up to 24 months when stored in a cool, dry place, providing laboratories with a reliable stock that reduces the need for frequent re‑ordering.

Designed for compatibility with automated plate‑pouring systems, the powder dissolves quickly and yields a homogenous solution that minimizes air bubbles and uneven surfaces. This feature streamlines high‑throughput workflows in core facilities and industrial screening labs, where consistency and speed are paramount. The medium also tolerates minor variations in pH and temperature, allowing flexibility in preparation protocols without compromising colony quality.

Storage guidelines recommend keeping the unopened pouch at 2‑8 °C, protected from moisture and direct sunlight. Once opened, the powder should be resealed tightly to prevent clumping and contamination. Under these conditions, the agar retains its nutritional profile and physical properties, ensuring that each plate produced meets the same high standards as the first batch.

RPI adheres to ISO 9001 quality management standards and complies with relevant regulatory requirements for laboratory reagents. Documentation includes a Certificate of Analysis for each lot, providing traceability and confidence for users who require validated materials for regulated research or product development.

The packaging includes clear labeling of batch number, expiration date, and safety information, facilitating inventory tracking and compliance with institutional safety protocols. A user‑friendly label design also aids quick identification in shared laboratory spaces, reducing the risk of mix‑ups with similar media types.

Usage

RPI YPD Agar is ideally suited for routine cultivation of Saccharomyces cerevisiae, Schizosaccharomyces pombe, and a variety of bacterial strains such as Escherichia coli and Bacillus subtilis. Researchers can pour plates at a concentration of 15 g/L agar, allowing for rapid solidification and easy handling. The medium supports both aerobic and microaerophilic conditions, making it versatile for experiments ranging from basic growth curves to complex phenotypic screenings.

In teaching laboratories, the agar provides a clear visual platform for students to observe colony morphology, perform streak‑plate techniques, and conduct antibiotic susceptibility tests. Its consistent nutrient profile ensures that experimental results are comparable across semesters, facilitating reliable assessment of student performance. The medium can also be supplemented with selective agents, such as antibiotics or specific carbon sources, to tailor growth conditions for specialized research projects.

To prepare the medium, dissolve the agar powder in distilled water, adjust the pH to 5.6–5.8 if required, and autoclave at 121 °C for 15 minutes. After cooling to 50 °C, pour into sterile Petri dishes and allow to solidify. The product’s stable formulation tolerates minor variations in pH and temperature, reducing preparation errors and ensuring consistent colony formation across multiple batches.

For large‑scale applications, the agar can be prepared in bulk volumes of up to 10 L, enabling the production of hundreds of plates with a single batch. This scalability is valuable for fermentation studies, high‑throughput screening, and industrial microbiology processes where uniform media performance is critical.

Common troubleshooting tips include verifying the autoclave cycle to avoid under‑ or over‑sterilization, ensuring the agar fully dissolves before cooling, and confirming that the poured plates are level to prevent uneven colony distribution. Adjusting the agar concentration slightly (e.g., 14 g/L) can improve plate firmness for specific downstream applications such as replica plating.

Compatibility with robotic plate handlers is enhanced by the agar’s smooth surface and consistent thickness, allowing seamless integration into automated workflows for colony picking, imaging, and downstream analysis. Users report reduced plate‑handling errors and increased throughput when pairing RPI YPD Agar with standard laboratory automation platforms.

Why Choose Us

RPI (Research Products International) has a long-standing reputation for delivering high‑quality microbiological media that meet stringent industry standards. The YPD Agar is manufactured in a GMP‑compliant facility, with each lot assigned a unique batch number for traceability. This commitment to quality control translates into reliable performance, minimizing variability that can compromise experimental outcomes.

Customer support is backed by a dedicated technical service team that can assist with media preparation, troubleshooting, and protocol optimization. Users benefit from rapid response times, detailed documentation, and access to a knowledge base that includes best‑practice guidelines for yeast and bacterial culture. This level of service ensures that laboratories can maintain productivity and focus on scientific discovery rather than logistical concerns.

RPI is committed to environmentally responsible manufacturing, employing recyclable packaging and minimizing waste during production. The agar powder is sourced from sustainably managed suppliers, and the manufacturing process adheres to ISO 14001 environmental management standards. Laboratories that prioritize green practices can confidently select this product, knowing it aligns with broader sustainability goals.

Our global distribution network ensures fast shipping to research institutions worldwide, with options for express delivery to minimize downtime. Regional warehouses maintain stock levels to provide immediate availability, reducing lead times and supporting time‑critical projects.

Numerous peer‑reviewed publications and case studies cite RPI YPD Agar as a preferred medium for high‑throughput yeast genetics, metabolic engineering, and antimicrobial testing. Customer testimonials highlight the medium’s consistency, ease of use, and the responsive technical assistance that helps resolve complex experimental challenges.

Key Features

• Consistent nutrient composition of peptone, dextrose, and yeast extract ensures reliable growth rates for a wide range of microorganisms.
• Ready‑to‑use 250 g pouch provides ample supply for dozens of plates, reducing the frequency of re‑ordering and inventory management effort.
• Low background fluorescence and minimal contaminants make the agar suitable for sensitive assays such as enzyme activity and fluorescence‑based measurements.
• Stable pH and solidification properties allow easy preparation and consistent plate quality across different laboratory environments.
• Dedicated technical support and comprehensive documentation help users troubleshoot and optimize protocols, ensuring smooth workflow integration.

FAQ

What is the optimal incubation temperature for cultures grown on RPI YPD Agar?

Most yeast strains, including Saccharomyces cerevisiae, thrive at 30 °C, while many bacterial species such as Escherichia coli grow best at 37 °C. The agar remains stable across this temperature range, and users can adjust incubation conditions based on the specific organism and experimental requirements.

Can I add antibiotics or selective agents to the YPD Agar?

Yes, the medium can be supplemented with antibiotics, such as ampicillin or nystatin, and other selective agents after autoclaving. Add the sterile filter‑sterilized solutions to the cooled agar (approximately 50 °C) to preserve activity, and mix gently before pouring plates.

How long can the prepared plates be stored before use?

Prepared plates can be stored at 4 °C for up to two weeks without significant loss of nutritional quality. For longer storage, seal the plates with parafilm to prevent dehydration, and consider adding a moisture‑retaining layer to maintain agar integrity.

Is the agar suitable for both yeast and bacterial strains?

RPI YPD Agar is formulated to support a broad spectrum of microorganisms. Its balanced composition provides the carbon and nitrogen sources required by many yeasts and also serves as a nutritious base for non‑fastidious bacteria. Users often employ it for mixed‑culture experiments and comparative growth studies.