Description
Product Overview
Innovating Science Nutrient Broth is a sterile, liquid growth medium formulated to support a wide range of bacterial species in laboratory research and quality control environments. The product is supplied as a 100 gram liquid concentrate that, when diluted, produces up to twelve liters of clear, nutrient‑rich broth suitable for use in test tubes, flasks, and bioreactors. The formulation contains balanced amounts of peptone, beef extract, and sodium chloride, providing essential carbon, nitrogen, and mineral sources that promote robust cell proliferation. The medium is free from preservatives and is packaged in a sealed, amber‑tinted container to protect against light degradation, ensuring consistent performance across multiple batches. Product dimensions are compact at two by two by four inches, weighing 4.44 ounces, making storage and handling straightforward for laboratory technicians.
Usage
This nutrient broth is intended for general‑purpose bacterial cultivation, including strain maintenance, growth curve studies, and preliminary screening assays. To prepare the medium, dissolve eight grams of the liquid concentrate into one liter of de‑ionized water, then bring the solution to a boil or autoclave at 121 °C for fifteen minutes to achieve sterility. After cooling, the broth can be aliquoted into sterile containers for inoculation. The medium supports aerobic and facultative anaerobic organisms, making it a versatile choice for microbiology labs, pharmaceutical development facilities, and academic research institutions. Users should follow standard aseptic techniques, such as working within a laminar flow hood and using sterile pipettes, to prevent contamination. The product’s stability allows for batch preparation, reducing the need for frequent reconstitution and minimizing workflow interruptions.
Why Choose Us
Innovating Science has a long history of supplying high‑quality laboratory reagents, and this nutrient broth reflects the company’s commitment to consistency and reliability. Each lot undergoes rigorous quality control testing, including sterility verification, pH measurement, and nutrient composition analysis, to guarantee that the medium meets defined performance criteria. The product’s discontinued status is false; it remains actively manufactured and supported, with a best‑seller rank indicating strong demand among professionals seeking dependable growth media. Customer reviews consistently rate the broth at five stars, highlighting its ease of preparation and reproducible results. In addition, the manufacturer provides responsive technical support, offering guidance on media preparation, troubleshooting, and optimization for specific bacterial strains. This level of service ensures that laboratories can rely on the product for both routine and specialized applications.
Key Features
- Provides a balanced nutrient profile that supports a broad spectrum of bacterial species.
- Yield of twelve liters per 100 g package reduces cost per experiment and simplifies inventory management.
- Liquid format eliminates the need for extensive dissolution time, enabling rapid preparation.
- Stable, amber‑tinted container protects the medium from light‑induced degradation.
- Dedicated technical support team assists with preparation protocols and strain‑specific recommendations.
FAQ
How do I sterilize the nutrient broth?
After diluting the concentrate with de‑ionized water, place the solution in autoclavable containers and run a standard autoclave cycle at 121 °C for fifteen minutes. This process eliminates contaminating microorganisms while preserving the integrity of the nutrients.
Can this broth be used for anaerobic bacteria?
Yes, the medium supports facultative anaerobes and can be adapted for strict anaerobes by adding reducing agents such as cysteine or by performing the preparation in an anaerobic chamber. The base formulation provides the essential nutrients required for growth.
What is the recommended storage condition for the unopened product?
Store the sealed container at room temperature, away from direct sunlight and moisture. The amber packaging helps maintain stability, and the product remains viable for at least two years when stored under these conditions.
Is the nutrient broth compatible with downstream applications such as DNA extraction?
Absolutely. The clear, low‑viscosity broth does not interfere with common downstream processes, including plasmid isolation, PCR amplification, and sequencing. After bacterial growth, cells can be harvested by centrifugation and processed using standard molecular biology protocols.
What troubleshooting steps should I take if I observe low bacterial growth?
First, verify that the pH of the prepared broth is within the optimal range (typically 7.0 ± 0.2). Ensure that the autoclave cycle was completed correctly and that the inoculum is viable. Adjusting incubation temperature, confirming aseptic technique, and checking for the presence of inhibitory substances in the water source can also improve growth outcomes.
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