HiMedia M002-100G Nutrient Broth, 100 g

Description

Product Overview

HiMedia M002-100G Nutrient Broth is a premium dehydrated culture medium formulated to meet the rigorous demands of modern life‑science laboratories. Packaged in a compact 100 g pouch, the product measures 4.75 × 2.5 × 2.5 inches and weighs just under 5 ounces, making storage and handling straightforward. The broth is composed of a balanced blend of peptone, beef extract, and sodium chloride, providing essential nutrients that support the rapid and uniform growth of a wide range of bacterial strains. Manufactured by HiMedia Laboratories, a trusted name in microbiological media since 1990, the broth adheres to strict quality‑control protocols, ensuring each batch meets the same high standards of purity and performance. Its dehydrated format offers a long shelf life, reducing the risk of contamination and allowing laboratories to prepare fresh broth on demand simply by reconstituting with sterile water. The product’s best‑seller rank of #29 in the Lab Natural Proteins category reflects its popularity among researchers who value consistency, reliability, and ease of use.

Usage

This nutrient broth is ideally suited for a variety of laboratory applications, including routine bacterial culture, antibiotic susceptibility testing, and the preparation of inocula for downstream assays such as enzyme activity measurements or protein expression studies. Researchers working in microbiology, molecular biology, and biotechnology can rely on the broth to produce robust growth curves, which are essential for accurate quantification of cell density and metabolic activity. The medium’s clear, non‑turbid appearance after reconstitution facilitates optical density measurements, while its defined composition minimizes background interference in biochemical assays. Because the broth is dehydrated, it can be stored at room temperature for extended periods, allowing labs to maintain a ready supply without the need for refrigeration. When reconstituted with sterile distilled water to a final volume of 100 ml, the broth yields a sterile, nutrient‑rich solution that can be autoclaved at 121 °C for 15 minutes to ensure sterility before use.

Why Choose Us

Choosing HiMedia’s Nutrient Broth means selecting a product backed by decades of expertise in microbiological media development. Each batch undergoes rigorous testing for sterility, pH stability, and nutrient content, guaranteeing reproducible results across experiments and between laboratories. The brand’s commitment to quality is reflected in its ISO‑9001 certification and adherence to international standards such as ASTM and USP. In addition to the high‑quality formulation, HiMedia provides responsive technical support, offering guidance on media preparation, troubleshooting growth issues, and optimizing protocols for specific bacterial strains. Customers benefit from a reliable supply chain that ensures timely delivery, and the company’s transparent labeling includes detailed specifications, lot numbers, and expiration dates for full traceability. Moreover, the product’s competitive pricing and bulk‑order options make it an economical choice for both academic institutions and commercial research facilities.

Key Features

• Compact 100 g pouch provides convenient storage and easy handling for laboratory workflows.
• Balanced nutrient composition supports rapid and uniform growth of diverse bacterial strains.
• Dehydrated format ensures long shelf life and reduces risk of microbial contamination.
• Clear solution after reconstitution enables accurate optical density measurements for quantitative assays.
• Dedicated technical support and detailed product documentation enhance user confidence and troubleshooting.

FAQ

What is the recommended reconstitution volume for this broth?

The standard protocol calls for adding 100 ml of sterile distilled water to the 100 g pouch, resulting in a 1 % (w/v) nutrient solution ready for autoclaving.

Can this broth be used for anaerobic bacterial cultures?

Yes, after autoclaving, the broth can be transferred to an anaerobic chamber or sealed containers to create an oxygen‑free environment suitable for anaerobic organisms.

Is the product compatible with automated liquid handling systems?

Absolutely. The broth’s powder form can be dispensed by most automated pipetting platforms, and the reconstituted solution is compatible with standard microplate readers and spectrophotometers.

How long can the reconstituted broth be stored before use?

Once prepared and sterilized, the broth should be stored at 2‑8 °C and used within 24 hours to maintain optimal nutrient integrity and prevent over‑growth of contaminating microbes.

What quality certifications does HiMedia hold for this product?

HiMedia Laboratories maintains ISO‑9001 certification and follows ASTM and USP guidelines for microbiological media, ensuring each batch meets stringent quality and safety standards.