HiMedia MV313-500G Todd Hewitt Hiveg Broth 500g Natural Protein

Description

Product Overview

The HiMedia MV313-500G Todd Hewitt Hiveg Broth is a premium laboratory medium designed to meet the demanding needs of microbiology research and quality‑control environments. Formulated with a precise balance of natural proteins, carbohydrates, and essential growth factors, this broth supports the robust cultivation of a wide range of fastidious microorganisms, including Streptococcus, Haemophilus, and other nutritionally demanding species. Each 500 g package delivers a consistent concentration of Todd Hewitt broth, ensuring reproducible results across multiple experiments and batches.

At the core of this product is the Todd Hewitt formulation, a classic medium renowned for its ability to promote rapid bacterial growth while maintaining physiological relevance. The inclusion of Hiveg (high‑intensity vegetable glycerol) enhances osmotic stability and provides an additional carbon source, further improving yield and cell viability. The natural protein component is derived from high‑purity sources, guaranteeing low endotoxin levels and minimal background interference, which is critical for downstream applications such as enzyme assays, vaccine development, and antimicrobial susceptibility testing.

Packaging is optimized for laboratory convenience: the product is supplied in a compact, resealable 500 g container with dimensions of 7.36 × 3.5 × 2.99 inches and a net weight of 1.36 lb. The container is designed to protect the broth from moisture and contamination, extending shelf life when stored at 2‑8 °C. Detailed labeling includes the model number MV313-500G, manufacturing date, and batch information, facilitating traceability and compliance with Good Laboratory Practice (GLP) standards.

Compatibility with standard laboratory protocols is a key advantage. The broth can be readily reconstituted with sterile distilled water to a final concentration of 10 g L⁻¹, or used directly in its powdered form for specialized applications that require higher concentration media. It is fully compatible with automated liquid handling systems, making it suitable for high‑throughput screening and large‑scale fermentation processes. The product meets the stringent quality criteria set by international regulatory bodies, including ISO 9001 and ISO 13485, ensuring that researchers receive a reliable, high‑performance medium every time.

Overall, the HiMedia MV313-500G Todd Hewitt Hiveg Broth combines scientific rigor, ease of use, and consistent performance, making it an indispensable component of any modern microbiology laboratory seeking to achieve accurate, reproducible, and high‑quality results.

Usage

This broth is ideally suited for a variety of laboratory settings, ranging from academic research labs to industrial quality‑control facilities. It excels in the cultivation of fastidious bacteria that require enriched nutrients for optimal growth, such as Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria gonorrhoeae. Researchers can employ the medium for routine culture, sub‑culturing, and preparation of inocula for downstream assays, including antibiotic susceptibility testing, virulence factor analysis, and vaccine antigen production.

To prepare the medium, dissolve the required amount of powder in sterile distilled water, typically 10 g per liter, and autoclave at 121 °C for 15 minutes. After cooling, the broth can be aliquoted into sterile containers for immediate use or stored at 2‑8 °C for up to six months. For applications requiring higher nutrient density, the broth can be concentrated up to 20 g L⁻¹, provided that appropriate sterility checks are performed.

In addition to traditional batch cultures, the broth performs exceptionally well in microtiter plate formats, enabling high‑throughput screening of antimicrobial compounds and genetic mutants. Its low background optical density allows for accurate spectrophotometric measurements, facilitating growth curve analysis and kinetic studies. The medium also supports the growth of yeast and certain fungal species when supplemented with additional nutrients, expanding its utility beyond bacterial cultures.

When used in conjunction with selective agents, such as antibiotics or specific carbon sources, the Todd Hewitt Hiveg Broth can be tailored to isolate and enrich target organisms from complex samples, including clinical specimens, environmental swabs, and food matrices. This flexibility makes it a valuable tool for diagnostic laboratories and food safety testing facilities.

Proper storage and handling are essential to maintain the broth’s integrity. Keep the unopened container in a cool, dry place, and avoid exposure to direct sunlight. Once opened, reseal the container tightly and store it in a refrigerator. Always use aseptic techniques when dispensing the medium to prevent contamination and ensure reproducible results.

Why Choose Us

HiMedia Laboratories has a long‑standing reputation for delivering high‑quality life‑science products that meet the exacting standards of modern research. Our Todd Hewitt Hiveg Broth is manufactured under strict GMP conditions, with each batch undergoing rigorous quality control testing, including sterility verification, endotoxin assessment, and nutrient composition analysis. This commitment to quality ensures that every gram of broth provides the same reliable performance, batch after batch.

Our state‑of‑the‑art production facilities are equipped with advanced filtration and lyophilization technologies, which preserve the activity of sensitive protein components while eliminating contaminants. The result is a medium that supports rapid bacterial growth without compromising the physiological relevance of the cultured organisms. Moreover, HiMedia’s extensive experience in microbiological media development allows us to fine‑tune the formulation for optimal performance across a broad spectrum of microorganisms.

Customer support is a cornerstone of our service philosophy. Our technical assistance team is available to help you with protocol optimization, troubleshooting, and custom formulation requests. Whether you need guidance on scaling up cultures, integrating the broth into automated workflows, or interpreting experimental data, our experts are ready to provide timely, knowledgeable assistance.

In addition to product excellence, HiMedia is committed to sustainability and responsible sourcing. The natural protein ingredients are derived from renewable resources, and our packaging materials are recyclable, reducing environmental impact while maintaining product integrity. By choosing HiMedia, you align your laboratory with a partner that values scientific advancement, reliability, and ecological stewardship.

Finally, our competitive pricing and reliable delivery schedule ensure that you receive the necessary quantities of Todd Hewitt Hiveg Broth when you need them, minimizing downtime and supporting uninterrupted research progress. Trust HiMedia for a seamless blend of quality, service, and value.

Key Features

• Consistent 500 g formulation delivers reliable growth for fastidious microorganisms.
• High‑purity natural protein ensures low endotoxin levels and minimal background interference.
• Hiveg component enhances osmotic stability and provides an additional carbon source.
• Compact, resealable packaging protects against moisture and contamination.
• Dedicated technical support and after‑sales service for protocol optimization.

FAQ

What types of microorganisms are best cultured with this broth?

The Todd Hewitt Hiveg Broth is especially suited for fastidious bacteria that require enriched nutrients, such as Streptococcus pneumoniae, Haemophilus influenzae, Neisseria species, and certain anaerobes. It also supports the growth of some yeast strains when supplemented with additional nutrients, making it a versatile choice for a wide range of microbiological applications.

How should the broth be reconstituted and stored for optimal performance?

To reconstitute, dissolve 10 g of the powder in 1 L of sterile distilled water, then autoclave at 121 °C for 15 minutes. After cooling, aliquot the broth into sterile containers and store at 2‑8 °C. The medium remains stable for up to six months when refrigerated and kept in a tightly sealed container. For longer storage, keep the unopened package in a cool, dry place away from direct sunlight.

Can this broth be used with automated liquid handling systems?

Yes, the powder form of the Todd Hewitt Hiveg Broth is compatible with most automated dispensing platforms. Its fine, free‑flowing texture allows for accurate weighing and dispensing, and the reconstituted liquid can be transferred using standard pipetting modules. This makes it ideal for high‑throughput screening, large‑scale fermentation, and other workflows that benefit from automation.

What quality certifications does HiMedia provide for this product?

HiMedia Laboratories manufactures the broth under GMP‑compliant conditions and adheres to ISO 9001 and ISO 13485 quality management standards. Each batch undergoes sterility testing, endotoxin quantification, and nutrient composition verification to ensure consistency and safety. Documentation, including certificates of analysis, is available upon request to support regulatory compliance and laboratory record‑keeping.

Is technical assistance available if I encounter issues with the broth?

Absolutely. HiMedia offers dedicated technical support staffed by experienced microbiologists and product specialists. Whether you need help optimizing culture conditions, troubleshooting unexpected growth patterns, or customizing the medium for specific applications, our team is reachable via phone, email, or online chat. We also provide detailed protocol guides and troubleshooting documents on our website to assist you in achieving the best possible results.